Project Overview

This tool is part of the CTCeek project for identifying circulating tumor cells.

CTCeek operates by leveraging the Seurat R package in several key steps:

• Data Upload: Upload your scRNA-seq expression matrix (e.g., RDS object, count matrix) into the app.
• Model Loading: Wait for the pre-computed reference model to load into the application. This may take a minute.
• Cell Classification: CTCeek applies the model using FindTransferAnchors and MapQuery to identify CTCs.
• Visualization & Export: Explore results through interactive tables and download findings for further research.

Software Description

CTCeek is a powerful R Shiny application designed to streamline the identification of Circulating Tumor Cell (CTCs) from single-cell RNA sequencing (scRNA-seq) data. It achieves this by employing a sophisticated, anchor-based framework to measure similarity between query cells and reference cell clusters. Leveraging robust methodologies from the Seurat R package, CTCeek facilitates the accurate classification of individual cells, identifying bona fide CTCs.

Test files and documentation are available on Zenodo: https://doi.org/10.5281/zenodo.17780234

Further information and original scripts can be found at https://github.com/PietroAnc/CTCeek